Directory of Methods

Nucleic Acids

DNA
Tail DNA for regular genotyping
Animal tail DNA for realtime PCR genotyping
DNA cleaning during cloning
DNA endblunting
Plasmid endofree maxiprep Qiagen
Plasmid miniprep Eppendorf
Plasmid miniprep Qiagen
DNA loading buffer (6 X)
Tail DNA extraction for regular genotyping - an alternative inexpensive method
Tail DNA extraction for genotyping - Another easy method
RNA
DEPC water
Total RNA extraction
Reverse transcription
Total RNA extraction (RNeasy plus kit)
PCR
Real time PCR
Regular PCR
PCR primer design
Rat ANF
PCR recipes
Mouse DGAT1
Cre recombinase
Human Adiponectin
Human beta Actin
Human GAPDH
Mouse Adiponectin
Mouse alpha cardiacmyosin heavy chain
Mouse AP2
Mouse Beta Actin
Mouse Elastase I
Mouse GAPDH
Mouse Glucagon
Mouse Insulin
Mouse LPL
Mouse Podocin
Mouse PPAR gamma2
Mouse spliced XBP 1
Mouse CD68
Mouse MCP1
Mouse Bip (GRP78)
Mouse ERdj4
Mouse CHOP
Mouse EDEM1
Mouse Sec61a
Mouse Leptin
Rat alpha-MHC
Rat beta-MHC
Rat BNF
Rat beta-Actin
Rat GAPDH
Rat 18s rRNA
Rat aSMA
Rat adiponectin (NM_144744)
Rat leptin
Rat spliced Xbp1
Rat Bip
Rat ERdj4
Rat CHOP
Rat EDEM1
Human beta-actin

Proteins

Quantitative Western blotting
Laemmli sample buffer (5X)
SDS-PAGE gel selection
Mouse tissue extraction
Protein precipitation
Nuclear and Cytosolic Fractionation
Nuclear/Cytosolic fractionation
Cytosolic and nuclear fractionation
Ponceau S solution

Antibody

Anti beta Actin antibody
Anti Cyclophilin antibody
Anti GFP antibody
Anti HA antibody
Anti human Adiponectin antibody
Anti human IkBA antibody
Anti human PPARg antibody
Anti mouse Adiponectin antibody
Anti Myc antibody
Anti human Akt1 antibody
Anti phospho-Akt(Ser473) antibody

Immunohistochemistry

Immunohistochemistry
Immunohistochemistry GFP
Immunohistochemistry Glucagon
Immunohistochemistry Glut2
Immunohistochemistry Insulin
Immunohistochemistry c-fos
Immunohistochemistry Mac-2
Immunofluorescence
Immunofluorescence 3T3-L1 adipocytes
Immunofluorescence Insulin Glut2
Immunofluorescence Insulin BrdU
Immunofluorescence Insulin Glucagon
Immunofluorescence beta Cell TUNEL
Immunofluorescence TUNEL staining heart
Immunofluorescence Insulin MafA

Virus

Lentivirus general
Lentivirus titration
Lentivirus production
Retrovirus packaging and infection

Mouse Genetics

Transgenic mouse general
Tamoxifen treatment

Mouse Genotyping Protocols

Mouse obob
Rosa26-rtTA-IRES-EGFP transgenic mouse
ATTAC transgenic mouse model
Adiponectin knockout mouse model
Cre transgenic mouse model
TRE-IkBa transgenic mouse model
TRE-IKK transgenic mouse model
Conditional knockout of MGAT1 mouse model
aMHC-tTA transgenic mouse model
MKR mouse model
Resistin knockout mouse model
Inducible Cre (MCM)

Metabolic Research

Oral glucose tolerance test (OGTT)
Pancreatic insulin content measurement
Insulin tolerance test (ITT)
3T3-L1 adipocyte differentiation
BrdU labeling of mouse beta cells
Intraperitoneal glucose tolerance test (IPGTT)
Glucose assay
Glucose measurement using a glucometer
PPAR gamma agonist treatment
ATTAC mouse model dimerizer administration
Mouse perfusion
In vivo insulin signaling

FACS

Bioinformatics ( coming soon )
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All you need to know when studying autophagy

Recently years have witnessed the explosive interests in autophagy in biology. Autophagy, originally considered as a simple catabolic process, has been implicated in various diseases including cancer, neurodegenerative disease, infectious disease and cardiovascular disease. Despite the rapid progression in deciphering functional roles of autophagy in pathology, the technical development to detect and quantify autophagy is lagging. Autophagy, as a dynamic process, is particularly difficult to quantify and the experimental data are frequently subjected to misinterpretations.

A recent review article in Cell provides all possible means to examine autophagy. The cons and pros of each method are discussed in great detail. The paper by Levine and colleagues sets a gold standard in the field of autophagy. Combined with another article in Autophagy by Klionsky and colleagues in 2008, these papers will help to clarify misconceptions and possibly serve as textbook in autophagy study. ... Read more highlights.