Directory of Methods

Nucleic Acids

DNA
Tail DNA for regular genotyping
Animal tail DNA for realtime PCR genotyping
DNA cleaning during cloning
DNA endblunting
Plasmid endofree maxiprep Qiagen
Plasmid miniprep Eppendorf
Plasmid miniprep Qiagen
DNA loading buffer (6 X)
RNA
DEPC water
Total RNA extraction
Reverse transcription
Total RNA extraction (RNeasy plus kit)
PCR
Real time PCR
Regular PCR
PCR primer design
PCR recipes
Mouse DGAT1
Cre recombinase
Human Adiponectin
Human beta Actin
Human GAPDH
Mouse Adiponectin
Mouse alpha cardiacmyosin heavy chain
Mouse AP2
Mouse Beta Actin
Mouse Elastase I
Mouse GAPDH
Mouse Glucagon
Mouse Insulin
Mouse LPL
Mouse Podocin
Mouse PPAR gamma2
Mouse spliced XBP 1
Mouse CD68
Mouse MCP1

Proteins

Quantitative Western blotting
Laemmli sample buffer (5X)
SDS-PAGE gel selection
Mouse tissue extraction

Antibody

Anti beta Actin antibody
Anti Cyclophilin antibody
Anti GFP antibody
Anti HA antibody
Anti human Adiponectin antibody
Anti human IkBA antibody
Anti human PPARg antibody
Anti mouse Adiponectin antibody
Anti Myc antibody
Anti human Akt1 antibody
Anti phospho-Akt(Ser473) antibody

Immunohistochemistry

Immunohistochemistry
Immunohistochemistry GFP
Immunohistochemistry Glucagon
Immunohistochemistry Glut2
Immunohistochemistry Insulin
Immunohistochemistry c-fos
Immunohistochemistry Mac-2
Immunofluorescence
Immunofluorescence 3T3-L1 adipocytes
Immunofluorescence Insulin Glut2
Immunofluorescence Insulin BrdU
Immunofluorescence Insulin Glucagon
Immunofluorescence beta Cell TUNEL
Immunofluorescence TUNEL staining heart
Immunofluorescence Insulin MafA

Virus

Lentivirus general
Lentivirus titration
Lentivirus production
Retrovirus packaging and infection

Mouse Genetics

Transgenic mouse general
Tamoxifen treatment

Mouse Genotyping Protocols

Mouse obob
Rosa26-rtTA-IRES-EGFP transgenic mouse
ATTAC transgenic mouse model
Adiponectin knockout mouse model
Cre transgenic mouse model
TRE-IkBa transgenic mouse model
TRE-IKK transgenic mouse model

Metabolic Research

Oral glucose tolerance test (OGTT)
Pancreatic insulin content measurement
Insulin tolerance test (ITT)
3T3-L1 adipocyte differentiation
BrdU labeling of mouse beta cells
Intraperitoneal glucose tolerance test (IPGTT)
Glucose assay
Glucose measurement using a glucometer
PPAR gamma agonist treatment
ATTAC mouse model dimerizer administration
Mouse perfusion
In vivo insulin signaling

FACS

GFTP positive cell FACS ( coming soon )
Double staining for macrophage and B cells ( coming soon )
Neuroscience ( coming soon )
Site News
New feature:
You can add comments on protocols now. Just go to any individual method page and click on the "Add a comment" link.

Highlight of the month
Negative VEGF

Angiogenesis is critical for tumor progression and development. Endothelial cells and vascular smooth muscle cells work together to form new vessels. VEGF and PDGF function through endothelial cells and VSMC during angiogenesis. However, the crosstalk between these two hormones is not clear. ... Read more highlights.