Introduction
The purpose of this site is to provide verified protocols for biology research.
As post-doctoral scholars/junior principle investigators, we have been looking for protocols that we can account for every day. Here, we put all the reliable protocols/reagents we have tested in this site. Unlike those methods described in scientific publications, our methods include great details and useful comments.
We have no intention to commercialize our methods and software. Our goal for this site is simple: make your work more productive and your life much easier, and encourage one another while we are in the same journey.
You can find the methods from the method directory or the search tool on the right.
Feature: please also try our customized, advanced biological method search as well as job search and you may find them cool tools.
New feature:
You can add comments on protocols now. Just go to any individual method page and click on
the "Add a comment" link.
All you need to know when studying autophagy
Recently years have witnessed the explosive interests in autophagy in biology. Autophagy, originally considered as a simple catabolic process, has been implicated in various diseases including cancer, neurodegenerative disease, infectious disease and cardiovascular disease. Despite the rapid progression in deciphering functional roles of autophagy in pathology, the technical development to detect and quantify autophagy is lagging. Autophagy, as a dynamic process, is particularly difficult to quantify and the experimental data are frequently subjected to misinterpretations.
A recent review article in Cell provides all possible means to examine autophagy. The cons and pros of each method are discussed in great detail. The paper by Levine and colleagues sets a gold standard in the field of autophagy. Combined with another article in Autophagy by Klionsky and colleagues in 2008, these papers will help to clarify misconceptions and possibly serve as textbook in autophagy study. ...
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