Rat ERdj4 (NM_012699)

rERdj4 5:ctc tcg gat gcc aat aga cg


rERdj4 3:ccg agt gtt ctg gtt ctg ac

Product: 168 bp

Recommended condition:

94C 5 min
94C 30 sec
58C 30 sec
72C 30 sec
X 35 cycles
72C 5 min
4C -


Recommended reaction:

18.5 ul H2O
2.5 ul PCR buffer (10X)
0.5 ul dNTP (10 mM each)
0.5 ul rERdj4 5 (10 uM)
0.5 ul rERdj4 3 (10uM)
0.5 ul Taq (5 U/ul)
2 ul DNA
--------
25 ul

These primers have been tested in cDNA samples for real-time PCR.


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