Cre transgenic mouse model

Cre 5:

GAT TTC GAC CAG GTT CGT TC

Cre 3:

GCT AAC CAG CGT TTT CGT TC

Product: 195 bp

Recommended condition:

94C 5 min
94C 30 sec
60C 30 sec
72C 30 sec
X 35 cycles
72C 5 min
4C -

Recommended reaction:

18.5 ul H2O
2.5 ul PCR buffer (10X)
0.5 ul dNTP (10 mM each)
0.5 ul Cre 5 (10 uM)
0.5 ul Cre 3 (10 uM)
0.5 ul Taq (5 U/ul)
2 ul DNA
--------
25 ul

These primers have been tested in cDNA samples and mouse tail DNA preparations for regular PCR and real-time PCR. These primers can be used for genotyping for all Cre transgenic/knockin mouse.


Comments

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Posted by guest Tue, 07 Dec 2010 02:50:14
Subject: not all cre lines are equal
we are working with different cre lines and most of the cre line shares same primers such as nes-cre, mx1-cre, lck-cre, rip-cre....
but recently I notice that vav-cre cannot use the same primer.
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