aMHC-tTA transgenic mouse model
aMHC-tTA mouse model was generated and characterized by Fishman and colleagues. The tetrocycline transactivator (tTA) is under the control of an alpha myosin heavy chain promoter which drives the expression specifically in cardiac myocytes. This transgenic mouse model may be combined with any Tre transgenic mouse model to confer spatio and tempo-specific expression. tTA is for Tet-off system only.
Ref: Yu Z, et al. Circ Res 2006.
tTA 5:
TCA GAC CGA GAT TTC TCC ATC CC
tTA 3:
GAA TTC AGG CTC GCC TGC AGT TGG
Product: 535 bp.
Recommended condition:
94C 5 min
94C 30 sec
56C 30 sec
72C 30 sec
X 35 cycles
72C 5 min
4C -
Recommended reaction:
18.5 ul H2O
2.5 ul PCR buffer (10X)
0.5 ul dNTP (10 mM each)
0.5 ul tTA 5 (10 uM)
0.5 ul tTA 3 (10 uM)
0.5 ul Taq (5 U/ul)
2 ul DNA
--------
25 ul
These primers have been tested in mouse tail DNA preparations to genotype aMHC-tTA animals.
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SIRT6 links glucose and lipid metabolism
SIRT1, a histone deacetylase, has drawn considerable attention due to its important roles in metabolic regulation and longevity. SIRT1 is induced by fasting and suppressed by feeding. SIRT1 increases glucose production and beta oxidation in liver to meet energy needs during food deprivation. Another member of SIRT family, SIRT6 follows similar expression patterns as SIRT1. The function of SIRT6 in metabolic regulation remains unknown.
Kim and colleagues found SIRT1 can regulate SIRT6 expression in liver. Specifically, SIRT1 functions together with FOXO3a and stimulates SIRT6 transcription during fasting. The activation of SIRT6 directly suppresses gene expression of metabolic enzymes involved in triglyceride synthesis and glycolysis in liver. Deficiency of SIRT6 causes increases of glucose utilization, reduced beta oxidation and as a result, fatty liver. More importantly, Kim et al found the expression of SIRT6 is decreased in human fatty liver samples which indicates SIRT6 may play critical roles in liver steatosis in clinical settings. This study landed in Cell Metabolism of this month.
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